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KMID : 0545120120220020023
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 2 p.23 ~ p.233
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Association of Colony Morphology with Coenzyme Q10 Production and Its Enhancement from Rhizobium radiobacter T6102W by Addition of Isopentenyl Alcohol as a Precursor
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Seo Myung-Ji
Kook Moo-Chang
Kim Soon-Ok
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Abstract
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Rhizobium radiobacter T6102 was morphologically purified by the aniline blue agar plates to give two distinct colonies; white smooth mucoid colony (T6102W) and blue rough colony (T6102B). The coenzyme Q10 (CoQ10) was produced just by T6102W, showing 2.0 mg/g of CoQ10 content, whereas the T6102B did not produce the CoQ10. All of the used CoQ10 biosynthetic precursors enhanced the CoQ10 production by T6102W. Specifically, the supplementation of 0.75 mM isopentenyl alcohol improved the CoQ10 concentration (19.9 mg/l) and content (2.4 mg/g) by 42% and 40%, respectively.
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KEYWORD
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Aniline blue, coenzyme Q10, isopentenyl alcohol, Rhizobium radiobacter
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